Suitability of peracetic acid for sterilization of media for mycoplasma cultures.

نویسندگان

  • P Wutzler
  • M Sprössig
  • H Peterseim
چکیده

The utility of peracetic acid for sterilization of serum and yeast extract additions to mycoplasma medium was studied by culturing six Mycoplasma species. Culture media containing additions that had been sterilized with peracetic acid proved to be as good as filtered components. The use of 0.05 to 0.1% peracetic acid is recommended to sterilize the serum and yeast extract additions since savings in time and equipment can be accomplished.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Vaporized Peracetic Acid (vpa) Sterilization

M in ne ap ol is , M N 5 54 47 VAPORIZED PERACETIC ACID (VPA) STERILIZATION Peracetic Acid as a Biocide REVOXTM sterilization technology uses a room temperature vapor composed of three active compounds; hydrogen peroxide (H2O2), acetic acid and peracetic acid (PAA). PAA is formed by the reaction of acetic acid and H2O2 with the addition of a catalyst; these compounds exist in equilibrium and th...

متن کامل

Suitability of MRS-bile Agar for the Selective Enumeration of Mixed Probiotic Bacteria in Presence of Mesophilic Lactic Acid Cultures and Yoghurt Bacteria

Measuring the viability of probiotic microorganisms in food products using plate count methodology is a common practice due to the simplicity (ease of performance), inexpensive and routine testing characters ofthis method. In present study, the suitability of de man rogosa and sharpe agar (MRS) bile agar medium forthe selective enumeration of mixed probiotic bacteria (Lactobacillus ...

متن کامل

Validation of peracetic acid as a sporicide for sterilization of working surfaces in biological safety cabinets

Objectives: This study aimed to validate the use of peracetic acid as sporicide agent to decontaminate the working surface of a laminar flow biological safety cabinet (BSC), as an alternative to glutaraldehyde, including the selection of the disinfecting agent, the method of application, and the contact time. Materials and methods: The test organism was the spore-forming bacterium Bacillus atro...

متن کامل

Isolation and detection of Mycoplasma pneumoniae from cell culture by culture and PCR.

Background: Mycoplasma pneumoniae is one of the major species of mycoplasmas which could contaminate cell cultures. Identification of M. pneuminae is significant because Mycoplasmas could contaminate and have unsuitable effect on the cell cultures. Rapid detection of these contaminations is so important and it could be significant role in preventing and controlling of contamination in cell cul...

متن کامل

Evaluation of the effectiveness of peracetic acid in the sterilization of dental equipment.

PURPOSE To evaluate the effectiveness of peracetic acid in the microbiological sterilisation of dental materials. METHODS Peracetic acid solution was evaluated at concentrations of 800, 1500 and 2500 ppm. At these concentrations, it was determined whether peracetic acid caused corrosion to dental instruments and induced cellular mutagenicity and cytotoxicity. In addition, the minimum inhibito...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Journal of clinical microbiology

دوره 1 3  شماره 

صفحات  -

تاریخ انتشار 1975